토쿄에서 개최되는 학술대회에 발표신청해서 2018년 11월 14-15일체 참석예정
발표 초록을 아래에 첨부하였다.

Distribution of beneficial bacteria in the intestines and Natural Killer Cell Activity after ENM ingestion of Bacillus subtilis AK Strain fermentation

                                            Seo Won Ryu¹ and Hyiung H. Lee²

     ¹RSW Dongeu Research Institute, Jecheon, Chungbuk, 27137, Korea(South)

     ²Department of Biological Sceicnes, Konkuk University, Seoul, 05029, Korea(South)

The purpose of this study was to investigate whether intestinal proliferation of beneficial intestinal bacteria and NK cell activity are promoted after ingesting Bacillus fermentation broth(ENM) for 8 weeks in the 16 subjects. Intestinal bacteria were identified by PCR amplification using specific 16S rRNA primers. The Bifidobacterium gene index(%)(gi%) increased to 69.53% in the test group. Lactobacillus gi% increased significantly (66.43% in the test)(p<.029). Clostridium gi% was significantly decreased after treatment (83.16% in the control and 67.76% in the test) (p<.077). Bacteroides gi% increased significantly to 20.87% in the test (p<.095). Prevotella gi% increased significantly 17.28% in the test(p<.005). The median bacteria increased significantly in the test(35.88%)(p<.001). NK cell activity of human ingested ENM was significantly higher in the test group (1796.37 pg/ml) (p<.004). Test groups were significantly increased by 1110.37pg/ml in the pre-test and 1796.37 pg/ml in the post-test  (p<.001). The bone matrix density was 0.062 g/cm³ in the test, which was increased by 0.003 g/cm³. The bone matrix content was 0.175 g in the control and 0.196 g in the test. The bone area was 3.19 cm² in the test, which was increased by 0.19 cm²_. After ingestion of the ENM, the number of beneficial bacteria increased and the number of harmful bacteria Clostridium tended to decrease. NK cell activity was significantly higher in the test group This suggests that ingestion of the Bacillus fermented beverage ENM has an effect on the proliferation of intestinal bacteria and NK cell activity.

Biography

Dr Hyung H. Lee has completed Masters in Microbiology from Yonsei University, Seoul, Korea, and Brigham Young University, UT, USA. and Ph.D. in Molecular Biology from University of Idaho, ID. USA. He currently teaches Naturopathic medicine at Department of Naturopathy, Dongbang Culture Graduate University and Kukje Theological University, South Korea. He has published several journal articles in the past.